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Food Production » Anaerobic Conditions and Yeast

What you'll learn this session

Study time: 30 minutes

  • How yeast carries out anaerobic respiration (fermentation)
  • The products of anaerobic respiration in yeast and their uses
  • How anaerobic conditions affect yeast metabolism
  • Industrial applications of yeast fermentation
  • Factors that influence fermentation rates
  • Comparing aerobic and anaerobic respiration in yeast

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Introduction to Anaerobic Conditions and Yeast

Yeast is a fascinating microorganism that can survive and thrive even when oxygen isn't available. This amazing ability makes yeast incredibly useful for humans - from baking bread to brewing beer! When yeast can't get oxygen, it switches to a different type of respiration called anaerobic respiration or fermentation.

Key Definitions:

  • Anaerobic respiration: Respiration that occurs without oxygen, producing less energy than aerobic respiration.
  • Fermentation: A type of anaerobic respiration where glucose is broken down to produce ethanol and carbon dioxide.
  • Yeast: Single-celled fungi that can respire both aerobically and anaerobically.
  • Ethanol: An alcohol produced during yeast fermentation.

🧞 What is Yeast?

Yeast are tiny single-celled organisms belonging to the fungi kingdom. The most common type used in food production is Saccharomyces cerevisiae. These microscopic powerhouses can switch between two types of respiration depending on whether oxygen is present or not.

Anaerobic Respiration in Yeast

When yeast finds itself in an environment without oxygen, it doesn't just give up and die. Instead, it cleverly switches to anaerobic respiration to keep producing the energy it needs to survive.

The Fermentation Process

During anaerobic respiration, yeast breaks down glucose (sugar) in a completely different way than it would with oxygen present. The process is much simpler but produces far less energy.

The fermentation equation:

Glucose → Ethanol + Carbon dioxide + Energy (ATP)

C₆H₁₂O₆ → 2C₂H₅OH + 2CO₂ + 2ATP

🍰 Glucose Input

Yeast uses glucose as its fuel source. This can come from added sugar, or from starches that have been broken down into simple sugars.

🍺 Ethanol Output

Ethanol (alcohol) is one of the main products. This is what makes alcoholic drinks alcoholic and also helps preserve food.

💨 Carbon Dioxide

CO₂ gas is released, which makes bread rise and creates the bubbles in sparkling drinks.

Energy Comparison

Anaerobic respiration in yeast produces only 2 ATP molecules per glucose molecule, compared to 38 ATP molecules produced during aerobic respiration. This is why yeast prefers oxygen when it's available - it's much more efficient!

Industrial Applications of Yeast Fermentation

The products of yeast fermentation - ethanol and carbon dioxide - are incredibly useful to humans. This is why we've been using yeast for thousands of years, even before we understood exactly how it worked.

Bread Making

In bread making, it's the carbon dioxide that's most important. When yeast ferments the sugars in flour, the CO₂ gas gets trapped in the dough, creating bubbles that make the bread light and fluffy.

🍞 The Bread Process

Yeast is mixed with flour, water and often a small amount of sugar. As the yeast ferments, CO₂ bubbles form throughout the dough. The heat from baking kills the yeast and sets the structure, leaving behind the familiar spongy texture of bread.

Alcoholic Beverages

For alcoholic drinks, it's the ethanol that's the star of the show. Different types of alcohol are made by fermenting different sugar sources.

🍺 Beer

Made by fermenting malted barley. The starches in barley are first converted to sugars, then yeast ferments these sugars into alcohol.

🍷 Wine

Made by fermenting grape juice. Grapes naturally contain sugars that yeast can directly ferment into alcohol.

🍸 Spirits

Made by fermenting various plant materials, then distilling to concentrate the alcohol content.

Factors Affecting Fermentation Rate

Several environmental factors can speed up or slow down the fermentation process. Understanding these helps us control fermentation for different purposes.

Temperature Effects

Temperature has a huge impact on how fast yeast works. Like most biological processes, fermentation is controlled by enzymes and enzymes are very sensitive to temperature.

🌡 Optimal Temperature

Most yeast works best at around 25-30°C. At this temperature, the enzymes involved in fermentation work at their maximum rate. Too cold and the process slows down dramatically. Too hot and the enzymes denature, stopping fermentation completely.

Sugar Concentration

The amount of sugar available affects how much fermentation can occur. More sugar generally means more fermentation, but there are limits.

pH Levels

Yeast prefers slightly acidic conditions (pH 4-6). If the environment becomes too acidic or too alkaline, fermentation slows down or stops.

Case Study: Commercial Bread Production

Large bakeries carefully control temperature, humidity and timing to ensure consistent bread quality. They often use special strains of yeast that work faster and produce more CO₂. The dough is kept at exactly the right temperature to optimise fermentation speed while maintaining flavour development.

Comparing Aerobic and Anaerobic Respiration

Understanding the differences between aerobic and anaerobic respiration helps explain why yeast behaves differently in different conditions.

Key Differences

💪 Aerobic Respiration

Requires: Oxygen
Products: CO₂ + Water + 38 ATP
Efficiency: Very high
Uses: Normal yeast growth and reproduction

🤖 Anaerobic Respiration

Requires: No oxygen
Products: Ethanol + CO₂ + 2 ATP
Efficiency: Low
Uses: Survival when oxygen is limited

Controlling Fermentation

In industrial food production, controlling fermentation is crucial for getting consistent, high-quality products.

Creating Anaerobic Conditions

To ensure fermentation occurs, oxygen must be excluded. This can be done by:

  • Using sealed containers
  • Covering mixtures with oil or plastic wrap
  • Using carbon dioxide to displace oxygen
  • Working in enclosed fermentation vessels

Stopping Fermentation

Sometimes we need to stop fermentation at the right moment:

  • Heat treatment: High temperatures kill yeast
  • Adding preservatives: Chemicals that inhibit yeast growth
  • Refrigeration: Cold temperatures slow fermentation dramatically
  • Filtration: Physically removing yeast cells

Environmental Impact

Yeast fermentation is considered environmentally friendly because it's a natural process that doesn't require harsh chemicals. The CO₂ produced is part of the natural carbon cycle and the process can use renewable plant materials as raw ingredients.

Modern Applications and Biotechnology

Today, scientists are finding new ways to use yeast fermentation beyond traditional food and drink production.

Biofuel Production

Yeast can ferment plant materials to produce ethanol for use as a renewable fuel. This biofuel can power cars and reduce dependence on fossil fuels.

Pharmaceutical Production

Genetically modified yeast can be used to produce medicines and vaccines through fermentation processes, making important drugs more affordable and accessible.

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