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    examBoard: Pearson Edexcel
    examType: IGCSE
    lessonTitle: Nitrates and Amino Acids
    
Biology - Plant Biology - Plant Nutrition - Nitrates and Amino Acids - BrainyLemons
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Plant Nutrition » Nitrates and Amino Acids

What you'll learn this session

Study time: 30 minutes

  • The role of nitrates in plant nutrition
  • How plants absorb and process nitrogen
  • The nitrogen cycle and its importance
  • How plants synthesise amino acids
  • The connection between nitrates and protein production
  • Environmental impacts of nitrogen fertilisers

Introduction to Plant Nutrition: Nitrates and Amino Acids

Plants need more than just carbon dioxide and water to grow. They also need minerals from the soil, especially nitrogen in the form of nitrates. These nitrates are essential for making amino acids, which are the building blocks of proteins. Without proteins, plants couldn't grow, repair themselves, or make enzymes for all their chemical reactions.

Key Definitions:

  • Nitrates: Compounds containing nitrogen and oxygen (NO₃⁻) that plants absorb from soil.
  • Amino acids: Organic compounds that contain an amino group (-NH₂) and are the building blocks of proteins.
  • Nitrogen fixation: The process of converting atmospheric nitrogen (N₂) into forms usable by living organisms.
  • Protein synthesis: The process by which cells build proteins using amino acids.

🌱 Why Plants Need Nitrogen

Nitrogen is a crucial element for plants because it's used to make:

  • Amino acids and proteins
  • DNA and RNA
  • Chlorophyll (the green pigment that captures light energy)
  • Plant hormones

Without enough nitrogen, plants develop yellow leaves (chlorosis) and stunted growth.

🔬 Forms of Nitrogen

Although nitrogen makes up about 78% of our atmosphere, plants can't use it directly in its gaseous form (N₂). Instead, they need nitrogen that has been 'fixed' into compounds like:

  • Nitrates (NO₃⁻) - most common form absorbed by plants
  • Ammonium ions (NH₄⁺) - can be used by some plants
  • Urea - found in fertilisers

The Nitrogen Cycle

The nitrogen cycle is the process by which nitrogen moves between the atmosphere, soil, water and living organisms. Understanding this cycle helps us see how plants get the nitrogen they need.

Key Stages of the Nitrogen Cycle

💫 Nitrogen Fixation

Atmospheric nitrogen (N₂) is converted to ammonia (NH₃) by:

  • Lightning (abiotic fixation)
  • Nitrogen-fixing bacteria in soil
  • Bacteria in root nodules of legumes (like peas and beans)
🚽 Nitrification

Ammonia is converted to nitrites (NO₂⁻) and then to nitrates (NO₃⁻) by nitrifying bacteria in the soil. Plants can absorb these nitrates through their roots.

🔃 Denitrification

Some bacteria convert nitrates back to nitrogen gas (N₂), which returns to the atmosphere. This happens mainly in waterlogged soils with little oxygen.

Case Study Focus: Legumes and Nitrogen Fixation

Leguminous plants like peas, beans and clover have a special relationship with nitrogen-fixing bacteria called Rhizobium. These bacteria live in nodules on the plants' roots and convert atmospheric nitrogen into ammonia that the plant can use. In return, the plant provides the bacteria with carbohydrates.

Farmers often plant legumes to naturally enrich soil with nitrogen - a practice called 'crop rotation'. After harvesting the legume crop, the next crop benefits from the extra nitrogen in the soil, reducing the need for artificial fertilisers.

How Plants Absorb and Use Nitrates

Plants absorb nitrates from the soil through their root hair cells by active transport. This means they use energy to move nitrates against the concentration gradient (from where there's less nitrate to where there's more).

🚀 Active Transport of Nitrates

Unlike carbon dioxide and water, nitrates don't just diffuse into plants - they need to be actively pumped in. This process:

  • Requires energy from respiration (ATP)
  • Uses special carrier proteins in cell membranes
  • Allows plants to accumulate nitrates even when soil levels are low
  • Is affected by temperature (slows down in cold weather)

From Nitrates to Amino Acids

Once inside the plant, nitrates undergo a transformation:

  1. Nitrates are reduced to nitrites
  2. Nitrites are further reduced to ammonium ions
  3. Ammonium ions combine with organic acids (made during photosynthesis)
  4. This forms amino acids - the building blocks of proteins

Amino Acid Synthesis and Protein Production

Amino acids are organic compounds containing carbon, hydrogen, oxygen and crucially, nitrogen. Plants synthesise amino acids by combining the nitrogen from nitrates with carbon compounds produced during photosynthesis.

From Amino Acids to Proteins

Once plants have made amino acids, they can link them together in long chains to form proteins. This process is called protein synthesis and happens in the ribosomes. Proteins serve many vital functions in plants:

  • Structural proteins give plants physical support
  • Enzymes catalyse chemical reactions
  • Transport proteins move substances across cell membranes
  • Storage proteins store nutrients, especially in seeds
  • Defensive proteins protect against pests and diseases

Did You Know? Nitrogen Deficiency Signs

When plants don't get enough nitrogen, they show specific symptoms:

  • Yellowing of older leaves first (chlorosis) as the plant moves nitrogen from old to new growth
  • Stunted growth and smaller leaves
  • Poor flowering and fruit production
  • Early leaf drop

Farmers and gardeners look for these signs to know when to add nitrogen fertilisers.

Environmental Impacts of Nitrogen Fertilisers

To boost crop yields, farmers often add nitrogen fertilisers to soil. While this helps plants grow, it can also cause environmental problems if not managed carefully.

💦 Eutrophication

When excess fertiliser washes into lakes and rivers (leaching), it can cause:

  • Algal blooms - rapid growth of algae on water surfaces
  • Reduced light penetration into water
  • Oxygen depletion as bacteria decompose dead algae
  • Death of fish and other aquatic organisms

🌍 Sustainable Nitrogen Use

Farmers can reduce nitrogen pollution by:

  • Using slow-release fertilisers
  • Testing soil before applying fertilisers
  • Planting cover crops to prevent leaching
  • Using crop rotation with legumes
  • Creating buffer zones near water sources

Summary: The Importance of Nitrates and Amino Acids

Nitrates are essential for plant growth because they provide the nitrogen needed to make amino acids. These amino acids are then used to build proteins, which are vital for all aspects of plant structure and function. The nitrogen cycle ensures that nitrogen moves between the atmosphere, soil and living organisms, making it available to plants.

Understanding how plants use nitrates and synthesise amino acids helps us grow better crops while protecting the environment. By managing nitrogen carefully, we can ensure plants get what they need without causing pollution in our waterways.

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