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Gas Exchange - Humans ยป Practical Investigation - Breathing

What you'll learn this session

Study time: 30 minutes

  • How to measure breathing rate and lung capacity practically
  • Understanding the effects of exercise on breathing
  • Using a spirometer to investigate lung function
  • Analysing data from breathing experiments
  • Safety considerations in breathing investigations
  • How to calculate and interpret breathing measurements

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Introduction to Practical Investigation - Breathing

Breathing is something we do automatically, but have you ever wondered how much air you actually breathe in and out? Or how your breathing changes when you exercise? Through practical investigations, we can measure and understand exactly how our respiratory system works. These experiments help us see the amazing efficiency of our lungs and how they adapt to different situations.

Key Definitions:

  • Breathing rate: The number of breaths taken per minute (measured as breaths/min).
  • Tidal volume: The volume of air breathed in and out during normal, quiet breathing (about 500ml).
  • Vital capacity: The maximum volume of air that can be expelled from the lungs after taking the deepest possible breath.
  • Spirometer: An instrument used to measure the volume of air inhaled and exhaled by the lungs.
  • Residual volume: The volume of air that remains in the lungs even after the most forceful expiration.

📈 Measuring Breathing Rate

The simplest breathing investigation involves counting breaths. Place one hand on your chest and count how many times it rises and falls in one minute. Normal resting breathing rate is 12-20 breaths per minute for teenagers. This increases dramatically during exercise as your body demands more oxygen.

Practical Methods for Investigating Breathing

There are several ways to investigate breathing practically, from simple observations to sophisticated equipment. Each method gives us different insights into how our respiratory system functions.

Method 1: Measuring Breathing Rate Changes

This investigation shows how breathing rate changes with activity level. You'll need a stopwatch and someone to help count breaths.

At Rest

Sit quietly for 2 minutes, then count breaths for 1 minute. Record the number. This is your resting breathing rate - typically 12-16 breaths per minute.

🏃 After Exercise

Do 2 minutes of step-ups or jumping jacks, then immediately count breaths for 1 minute. You'll find this number is much higher - often 25-40 breaths per minute.

📊 Recovery

Continue measuring every minute until breathing returns to resting rate. This usually takes 3-5 minutes, showing how efficiently your body recovers.

Why Does Breathing Rate Increase?

During exercise, your muscles work harder and need more oxygen to release energy from glucose. Your breathing rate increases to take in more oxygen and remove the extra carbon dioxide produced. Your heart rate also increases to pump this oxygen-rich blood around your body faster.

Method 2: Using a Spirometer

A spirometer is the gold standard for measuring lung volumes. Modern digital spirometers are safe and easy to use in schools, giving accurate measurements of different lung capacities.

What a spirometer measures:

  • Tidal Volume: Normal breathing volume (about 500ml)
  • Inspiratory Reserve Volume: Extra air you can breathe in after normal inspiration (about 3000ml)
  • Expiratory Reserve Volume: Extra air you can breathe out after normal expiration (about 1100ml)
  • Vital Capacity: Maximum air you can breathe out after deepest breath (about 4600ml for teenagers)

Investigating the Effects of Exercise

One of the most interesting investigations is seeing how exercise affects not just breathing rate, but also the depth of breathing and recovery time.

💪 Exercise Investigation

Measure breathing rate before, during and after different intensities of exercise. Light exercise might increase breathing to 20 breaths/min, while intense exercise can push it to 40+ breaths/min. The fitter you are, the quicker your breathing returns to normal.

Method 3: Measuring Lung Capacity with Simple Equipment

You can estimate lung capacity using a large plastic bottle, tubing and water - though this is less accurate than a spirometer.

Equipment needed:

  • Large plastic bottle (2-3 litres)
  • Rubber tubing
  • Large bowl or sink
  • Measuring cylinder
  • Marker pen

Method:

  1. Fill the bottle completely with water
  2. Turn it upside down in a bowl of water (keeping the neck under water)
  3. Insert tubing into the bottle neck
  4. Take the deepest breath possible and blow all air through the tube
  5. Measure the volume of water displaced - this approximates your vital capacity

Case Study: Athlete vs Non-Athlete

A study comparing a trained swimmer with a non-athletic student showed fascinating differences. The swimmer had a vital capacity of 5200ml compared to 4100ml for the non-athlete. More importantly, the swimmer's breathing rate only increased to 28 breaths/min during intense exercise, while the non-athlete reached 42 breaths/min. The swimmer also recovered to resting rate in just 2 minutes compared to 6 minutes for the non-athlete.

Safety and Accuracy in Breathing Investigations

When investigating breathing, safety must come first. Never push anyone beyond their comfort zone and always have someone supervising exercise activities.

Safety Guidelines

  • Never force anyone to exercise if they feel unwell
  • Stop immediately if someone feels dizzy or short of breath
  • Use individual mouthpieces for spirometers to prevent infection
  • Clean all equipment between users
  • Have someone trained in first aid present during investigations
  • Allow proper rest periods between measurements

Improving Accuracy

Take multiple measurements and calculate averages. Breathing can vary due to nervousness, time of day, or recent activity. The more data you collect, the more reliable your results will be. Always record conditions like temperature and humidity as these can affect measurements.

Analysing and Interpreting Results

The real learning comes from analysing what your measurements mean. Look for patterns in your data and try to explain the biological reasons behind what you observe.

What to Look For

When analysing breathing data, consider these factors:

  • Individual variation: People of similar age and fitness can have different lung capacities
  • Recovery patterns: How quickly breathing returns to normal indicates fitness level
  • Exercise intensity: Harder exercise causes proportionally greater increases in breathing rate
  • Adaptation: Regular exercise improves both lung capacity and efficiency

Real-World Applications

These investigations mirror tests used in medicine and sports science. Doctors use spirometry to diagnose conditions like asthma and COPD. Sports scientists use similar tests to assess athlete performance and design training programmes. Understanding how to measure and interpret breathing data is a valuable skill beyond the classroom.

Common Mistakes and How to Avoid Them

Even simple breathing investigations can go wrong if you're not careful. Here are the most common mistakes and how to avoid them.

Timing Errors

Always use a stopwatch and count for a full minute. Counting for 15 seconds and multiplying by 4 introduces errors because breathing isn't perfectly regular.

🔍 Observer Effects

People breathe differently when they know they're being watched. Try to be discreet when counting, or use equipment that measures automatically.

📋 Poor Recording

Write down measurements immediately. It's easy to forget numbers, especially when taking multiple readings from different people.

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